Restriction Site Mapping Of Λ Phage Dna - 3505 Words

FIFE COLLEGE Restriction Site Mapping in ÃŽ » Phage DNA Daniel Richards 0612924 Table of Contents Introduction 3 Aims and Objectives 7 Methods 8 Results 13 Discussion 14 Sources of Error 15 Conclusion 16 References 17 Introduction Lambda phage, also known as enterobacteria phage ÃŽ », is a bacteriophage that infects Escherichia coli. The lambda phage has the capability to reside in the genome of its host through lysogeny or to enter a lytic phase, during which it lyses the cell to produce offspring.[1] The phage consists of a capsid, a tail and tail fibres with the head containing the phage s double-strand linear DNA genome. The genome contains 48,490 base pairs with 12-base single strand segments at both 5 ends. However the cos site circularizes the DNA in the host cytoplasm, therefore in its circular form is 48,502 base pairs in length. The lytic cycle is the life cycle that more commonly occurs after most infections. It begins with the attachment of the phage to the host cell allowing it to inject its DNA into the cell. Following this nucleic acid from the phage is replicated causing the phage s genes to be expressed which allows for the production of phage proteins. These proteins are then assembled into phage particles which are released when the cell undergoes lysis. This lysis is mediated by genes S, R, Rz and Rz, these genes are shown in the diagram below, which work together to break down the cell wall of the host bacterium. Whilst this mode ofShow MoreRelatedLocating The Xhoi Recognition Site On Lambda Dna Using1704 Words   |  7 PagesLocating the XhoI Recognition Site on Lambda DNA Using a HindIII and XhoI double digest Haleigh Wood Abstract Restriction enzymes cut DNA at certain sites to create multiple DNA fragments. Restriction enzyme HindIII has known DNA fragment lengths and recognition sites when digesting lambda DNA, while the lambda DNA recognition site for restriction enzyme XhoI is unknown. The goal of this study is to determine the lambda recognition site of XhoI by comparing a HindIII digest and a HindIII and XhoIRead MoreBiological Molecules Like Nucleic Acids And Polysachharides2245 Words   |  9 Pagessource. Then amplification of these isolated genes is done. Amplification is done by the insertion of the target gene into a specified vector. Vector is also a DNA sequence molecule. The vector that has foreign gene in now a recombinant DNA. It replicates in cells after insertion in living cells. As vector gets replicates, the recombinant DNA (rDNA) also gets replicate. This gene expresses the protein. This expressed protein is then purified and further analysis can be done (Ausubel et al., 2002).Read MoreT he Viological Structure Of G-Protein Coupled Receptorss1463 Words   |  6 Pagesillustrates the outward movement of a GPCR in the intracellular region to allow binding of GÉ‘. Simultaneously, the extracellular region move inward to form the closed state (high affinity); this impairs the dissociation of the agonist from the ligand-binding site. Experiment 1 facilitated our understanding of the molecular nature of GPCRs by providing experimental evidence for the Ternary Complex model. This model explains how ligand binding leads to a conformational change in the GPCR and a downstream signalingRead MoreMolecular Plant Microbe Interaction : Evolution Of Plant Pathogens Essay2585 Words   |  11 Pageslocalized cell death at the site of infection. These R proteins can also recognise the effector proteins indirectly through a mechanism called The Guard Hypothesis. Natural selection then helps the pathogen escape the HR by divergence or by acquiring additional effectors (Jones et al., 2006). Hence, in order to invade the plant further, the pathogens should undergo evolution. Whole Genome Analysis: Whole genome sequencing has served as a tool in determining the complete DNA of a particular organismRead MoreCell Biology Final Essay30093 Words   |  121 Pages_______ in eukaryotic cells. A) ribosomes B) oxidative phosphorylation C) DNA molecules -D) a nucleus 2. Cytoplasmic organelles are - A) absent in prokaryotic cells; present in eukaryotic cells. B) present in both prokaryotic and eukaryotic cells. C) present in prokaryotic cells; absent in eukaryotic cells. D) absent in both prokaryotic and eukaryotic cells. 3. Eukaryotic chromosomes contain _______ DNA molecules. A) single linear B) single circular -C) multiple linear